A simple, precise, and accurate high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of Ofloxacin (OFL) and Cefpodoxime Proxetil (CPD) in their combined tablet dosage form has been developed and validated. Chromatographic separation was carried out on silica gel 60 GF254 HPTLC plates with Ethanol-Ethyl Acetate-Water-Triethylamine (5:3.2:1.8:0.15 v/v/v/v) as mobile phase. Densitometric quantitation was performed at 290 nm. Well-resolved bands were obtained with Rf values 0.24 and 0.61 for Ofloxacin and Cefpodoxime Proxetil respectively. The method was validated for precision, accuracy and specificity. The calibration curve was found to be linear in the concentration range of 200-1000 ng/band for OFL and CPD respectively. Correlation co-efficient is 0.9997 and 0.9990 for OFL and CPD respectively. The method is selective and specific, with potential application in pharmaceutical analysis of these drugs in combined tablet dosage form.
Loading....